Relevant Publications:
Yuan S, Yin X, Meng X, Chan JFW, Ye ZW, Riva L, et al. Clofazimine broadly inhibits coronaviruses including SARS-CoV-2. Nature. 593(7859):418-23.(2021)
Keung, W., Ren, L., Sen Li, Wong, A. O., Chopra, A., Kong, C. W., Tomaselli G. F., Chen, C. S., Li, R. A. Non-cell autonomous cues for enhanced functionality of human embryonic stem cell-derived cardiomyocytes via maturation of sarcolemmal and mitochondrial K(ATP) channels. Sci Rep. 6, 34154 (2016).
Poon, E., Keung, W., Liang, Y., Ramalingam, R., Yan, B., Zhang, S., Chopra, A., Moore, J., Herren, A., Lieu, D. K., Wong, H. S., Weng, Z., Wong, O. T., Lam, Y. W., Tomaselli, G. F., Chen, C., Boheler, K. R. & Li, R. A. Proteomic Analysis of Human Pluripotent Stem Cell-Derived, Fetal, and Adult Ventricular Cardiomyocytes Reveals Pathways Crucial for Cardiac Metabolism and Maturation. Circ Cardiovasc Genet 8, 427–436 (2015).
Zhang, S., Poon, E., Xie, D., Boheler, K. R., Li, R. A., Wong, H. S. Consensus comparative analysis of human embryonic stem cell-derived cardiomyocytes. PLoS One. 10, e0125442 (2015).
Karakikes I., Stillitano F., Nonnenmacher M., Tzimas C., Sanoudou D., Termglinchan V., Kong C. W., Rushing S., Hansen J., Ceholski D., Kolokathis F., Kremastinos D.,Katoulis A., Ren L., Cohen N., Gho J. M., Tsiapras D., Vink A., Wu J. C., Asselbergs F. W., Li R. A., Hulot J. S., Kranias E. G., Hajjar R. J. Correction of human phospholamban R14del mutation associated with cardiomyopathy using targeted nucleases and combination therapy. Nat Commun. 6, 6955 (2015).
Chen, G., Li, S., Karakikes, I., Ren, L., Chow, M. Z., Chopra, A., Keung, W., Yan, B., Chan, C. W., Costa, K. D., Kong, C. W., Hajjar, R. J., Chen, C. S., Li, R. A. Phospholamban as a crucial determinant of the inotropic response of human pluripotent stem cell-derived ventricular cardiomyocytes and engineered 3-dimensional tissue constructs. Circ Arrthyhm Electrophysiol. 8, 193-201 (2015).
Li, R. A. Cardiovascular regeneration. Stem Cell Res Ther. 5, 141 (2014).
Weng, Z., Kong, C.-W., Ren, L., Karakikes, I., Geng, L., He, J., Chow, M. Z. Y., Mok, C. F., Chan, H. Y. S., Webb, S. E., Keung, W., Chow, H., Miller, A. L., Leung, A. Y. H., Hajjar, R. J., Li, R. A. & Chan, C. W. A simple, cost-effective but highly efficient system for deriving ventricular cardiomyocytes from human pluripotent stem cells. Stem Cells Dev. 23, 1704–1716 (2014).
Keung, W., Boheler, K. R., Li, R. A., Developmental cues for the maturation of metabolic, electrophysiological and calcium handling properties of human pluripotent stem cell-derived cardiomyocytes. Stem Cell Res Ther. 5, 17, (2014).
Karakikes, I., Senyel, G. D., Hansen, J., Kong, C.-W., Azeloglu, E. U., Stillitano, F., Lieu, D. K., Wang, J., Ren, L., Hulot, J.-S., Iyengar, R., Li, R. A. & Hajjar, R. j. Small molecule-mediated directed differentiation of human embryonic stem cells toward ventricular cardiomyocytes. Stem Cells Transl. Med. 3, 18–31 (2014).
Li, S., Cheng, H., Tomaselli, G. F., Li, R. A. Mechanistic basis of excitation-contraction coupling in human pluripotent stem cell-derived ventricular cardiomyocytes revealed by Ca2+ spark characteristics: direct evidence of functional Ca2+-induced Ca2+ release. Heart Rhythm. 11, 133-140 (2014).
Li, S., Chen, G., Li, R. A. Calcium signalling of human pluripotent stem cell-derived cardiomyocytes. J Physiol. 591, 5279-5290 (2013).
Poon, E., Yan, B., Zhang, S., Rushing, S., Keung, W., Ren, L., Lieu, D. K., Geng, L., Kong, C. W., Wang, J., Wong H. S., Boheler, K. R., Li, R. A. Transcriptome-guided functional analyses reveal novel biological properties and regulatory hierarchy of human embryonic stem cell-derived ventricular cardiomyocytes crucial for maturation. PLoS One. 8, e77784 (2013).
Chow, M. Z., Geng, L., Kong, C. W., Keung, W., Fung, J. C., Boheler, K. R., Li, R. A. Epigenetic regulation of the electrophysiological phenotype of human embryonic stem cell-derived ventricular cardiomyocytes: insights for driven maturation and hypertrophic growth. Stem Cells Dev. 22, 2678-2690 (2013).
Chow, M., Boheler, K. R., Li, R. A. Human pluripotent stem cell-derived cardiomyocytes for heart regeneration, drug discovery and disease modelling: from the genetic, epigenetic, and tissue modeling perspective. Stem Cell Res Ther. 4, 97 (2013).
Lieu, D. K., Fu, J. D., Chiamvimonvat, N., Tung, K. C., McNerney, G. P., Huser, T., Keller, G., Kong, C. W., Li, R. A. Mechanism-based facilitated maturation of human pluripotent stem cell-derived cardiomyocytes. Circ Arrhythm Electrophysiol. 6, 191-201 (2013).
Fu, J. D., Rushing, S. N., Lieu, D. K., Chan, C. W., Kong, C. W., Geng, L., Wilson, K. D., Chiamvimonvat, N., Boheler, K. R., Wu, J. C., Keller, G., Hajjar, R. J., Li, R. A. Distinct roles of microRNA-1 and -499 in ventricular specification and functional maturation of human embryonic stem cell-derived cardiomyocytes. PLoS One. 6, e27417 (2011).
Wilson, K. D., Hu, S., Venkatasubrahmanyam, S., Fu, J. D., Sun, N., Abilez, O. J., Baugh, J. J., Jia, F., Ghosh, Z., Li, R. A., Butte, A. J., Wu, J. C. Dynamic microRNA expression programs during cardiac differentiation of human embryonic stem cells: role for miR-499. Circ Cardiovasc Genet. 3, 426-435 (2010).
Fu, J. D., Jiang, P., Rushing, S., Liu, J., Chiamvimonvat, N., Li, R. A. Na+/Ca2+ exchanger is a determinant of excitation-contraction coupling in human embryonic stem cell-derived ventricular cardiomyocytes. Stem Cells Dev. 19, 773-782 (2010).
Liu, J., Lieu, D. K., Siu, C. W., Fu, J. D., Tse, H. F., Li, R. A. Facilitated maturation of Ca2+ handling properties of human embryonic stem cell-derived cardiomyocytes by calsequestrin expression. Am J Physiol Cell Physiol. 297, C152-159 (2009).
Lieu, D. K., Liu, J., Siu, C. W., McNerney, G. P., Tse, H. F., Abu-Khalil, A., Huser, T., Li, R. A. Absence of transverse tubules contributes to non-uniform Ca(2+) wavefronts in mouse and human embryonic stem cell-derived cardiomyocytes. Stem Cells Dev. 18, 1493-1500 (2009).
Chan, J. W., Lieu, D. K., Huser, T., Li, R. A. Label-free separation of human embryonic stem cells and their cardiac derivatives using Raman spectroscopy. Anal Cham. 81, 1324-1331 (2009).
Liu J., Fu J. D., Siu C. W., Li R. A. Functional sarcoplasmic reticulum for calcium handling of human embryonic stem cell-derived cardiomyocytes: insights for driven maturation. Stem Cells. 12, 3038-44 (2007).
Wang K., Xue T., Tsang S. Y., Van Huizen R., Wong C. W., Lai K. W., Ye Z., Cheng L., Au K. W., Zhang J., Li G. R., Lau C. P., Tse H. F., Li R. A. Electrophysiological properties of pluripotent human and mouse embryonic stem cells. Stem Cells. 10, 1526-34 (2005).
